Microfluidic Flow Electroporation
CyteQuest delivers mRNA, DNA, and CRISPR RNP payloads into cells in continuous flow — pairing high transfection efficiency with the gentle, uniform field that keeps cells healthy, from R&D batches to commercial scale.
The challenge
Traditional electroporation requires tedious optimization, lacks scalability, and offers users little flexibility. CyteQuest's microfluidic platform is designed to address these failures.
CyteQuest's platform is designed to automate optimization to achieve highly efficient transfection while preserving cell viability. Importantly, users can seamlessly scale to any processing volume to support applications from R&D through production.
Why microfluidic flow
Maximize delivery of DNA, mRNA, or CRISPR-Cas9 RNPs for robust, reproducible genetic modification across diverse cell types.
Efficient, automated optimization eases difficulty in identifying parameters that minimize cellular stress, leaving more healthy, functional cells able to expand and perform after transfection.
Continuous flow produces consistent, predictable results from small R&D batches to commercial volumes — no re-optimization between scales.
Core technology
A uniform electric field opens temporary pores in the cell membrane, cargo enters, and the membrane reseals — all while cells move through the channel in continuous flow.
R&D → Clinical
Scale between R&D and production volumes without re-optimization.
Rapidly sweep through transfection parameters in the CytoZap Scan using small volumes of cells and reagents. Directly transfer these parameters to the CytoZap Max for large-volume, production scale delivery in a closed system for identical performance without re-optimization.
Waveform control
CyteQuest's platform applies an arbitrary, time-varying voltage waveform — not a fixed pulse. Every parameter can be tuned and optimized for a specific cell type and cargo combination, then reused for reproducible delivery.
Choose a waveform type and drag the sliders to see how each parameter reshapes the continuously cycling waveform. The platform can apply this level of control to match any cell and cargo combination.
Applications
Performance data for the following applications:
Shape the future of cell engineering
Partner with CyteQuest to bring gentle, high-efficiency flow electroporation to your pipeline — optimizing delivery for any cell and cargo, preserving viability, and scaling from R&D to production.
Partner with CyteQuest